PURIFICATION OF THE ESCHERICHIA-COLI INTEGRATION HOST FACTOR (IHF) IN ONE CHROMATOGRAPHIC STEP

被引：15

作者：

FILUTOWICZ, M

GRIMEK, H

APPELT, K

机构：

[1] UNIV WISCONSIN,CTR BIOTECHNOL,MADISON,WI 53706

[2] AGOURON PHARMACEUT,SAN DIEGO,CA 92121

来源：

GENE | 1994年 / 147卷 / 01期

关键词：

DNA-BINDING/BENDING PROTEIN; PROTEIN ISOLATION; IHF;

D O I：

10.1016/0378-1119(94)90056-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The integration host factor (IHF) participates in a diverse array of DNA transactions such as replication, recombination and gene expression. We describe a fast and very efficient isolation procedure which yields highly purified IHF in one chromatographic step.

引用

页码：149 / 150

页数：2

共 7 条

[1]

DELLIS S, 1992, J BIOL CHEM, V267, P24426

[2]

FILUTOWICZ M, 1991, J BIOL CHEM, V266, P24077

[3] THE INTEGRATION HOST FACTOR OF ESCHERICHIA-COLI BINDS TO MULTIPLE SITES AT PLASMID R6K GAMMA-ORIGIN AND IS ESSENTIAL FOR REPLICATION [J].