THE PROTEOLYTIC PROCESSING SITE OF THE PRECURSOR OF LYSYL OXIDASE

被引：71

作者：

CRONSHAW, AD ^{[1
]}

FOTHERGILLGILMORE, LA ^{[1
]}

HULMES, DJS ^{[1
]}

机构：

[1] UNIV EDINBURGH,DEPT BIOCHEM,EDINBURGH EH8 9DX,MIDLOTHIAN,SCOTLAND

来源：

BIOCHEMICAL JOURNAL | 1995年 / 306卷

基金：

英国惠康基金;

关键词：

D O I：

10.1042/bj3060279

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The precise cleavage site of the N-terminal propeptide region of the precursor of lysyl oxidase has not yet been established, due to N-terminal blocking of the mature protein. Using a combination of peptide fragmentation, amino acid sequencing, time-of-flight m.s. and partial chemical unblocking procedures, it is shown that the mature form of lysyl oxidase begins at residue Asp-169 of the precursor protein (numbered according to the human sequence). The cleavage site is 28 residues to the C-terminal side of the site previously suggested on the basis of apparant molecular mass by SDS/PAGE, with the consequence that the two putative, N-linked glycosylation sites and the position of the Arg/Gln sequence polymorphism are now all in the precursor region.

引用

页码：279 / 284

页数：6

共 37 条

[1]

AITKEN A, 1990, IDENTIFICATION PROTE, P55

[2] THE AMINO-ACID-SEQUENCE OF THE DIHEME CYTOCHROME-C4 FROM THE BACTERIUM AZOTOBACTER-VINELANDII [J].

AMBLER, RP ;

DANIEL, M ;

MELIS, K ;

STOUT, CD .

BIOCHEMICAL JOURNAL, 1984, 222 (01) :217-227

[3]

ARLIN SM, 1988, BIOCHEMISTRY-US, V27, P7979

[4] TRAMP (TYROSINE RICH ACIDIC MATRIX PROTEIN), A PROTEIN THAT COPURIFIES WITH LYSYL OXIDASE FROM PORCINE SKIN - IDENTIFICATION OF TRAMP AS THE DERMATAN SULFATE PROTEOGLYCAN-ASSOCIATED 22K EXTRACELLULAR-MATRIX PROTEIN [J].

CRONSHAW, AD ;

MACBEATH, JRE ;

SHACKLETON, DR ;

COLLINS, JF ;

FOTHERGILLGILMORE, LA ;

HULMES, DJS .

MATRIX, 1993, 13 (03) :255-266

[5] A RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM RESULTS IN A NONCONSERVATIVE AMINO-ACID SUBSTITUTION ENCODED WITHIN THE 1ST EXON OF THE HUMAN LYSYL OXIDASE GENE [J].

CSISZAR, K ;

MARIANI, TJ ;

GOSIN, JS ;

DEAK, SB ;

BOYD, CD .

GENOMICS, 1993, 16 (02) :401-406

[6]

DRAPEAU GR, 1990, J BIOL CHEM, V255, P839

[7] THE MECHANISM OF N-TERMINAL ACETYLATION OF PROTEINS [J].

DRIESSEN, HPC ;

DEJONG, WW ;

TESSER, GI ;

BLOEMENDAL, H .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1985, 18 (04) :281-325

[8] MOLECULAR-CLONING OF HUMAN LYSYL OXIDASE AND ASSIGNMENT OF THE GENE TO CHROMOSOME-5Q23.3-31.2 [J].

HAMALAINEN, ER ;

JONES, TA ;

SHEER, D ;

TASKINEN, K ;

PIHLAJANIEMI, T ;

KIVIRIKKO, KI .

GENOMICS, 1991, 11 (03) :508-516

[9] EVIDENCE THAT GLUTATHIONE S-TRANSFERASE-B1B1 AND S-TRANSFERASE-B2B2 ARE THE PRODUCTS OF SEPARATE GENES AND THAT THEIR EXPRESSION IN HUMAN-LIVER IS SUBJECT TO INTER-INDIVIDUAL VARIATION - MOLECULAR RELATIONSHIPS BETWEEN THE B1-SUBUNIT AND B2-SUBUNITS AND OTHER ALPHA-CLASS GLUTATHIONE S-TRANSFERASE [J].

HAYES, JD ;

KERR, LA ;

CRONSHAW, AD .

BIOCHEMICAL JOURNAL, 1989, 264 (02) :437-445

[10]

HENRY GD, 1982, FEBS LETT, V14, P11

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