CARBOXYL-TERMINAL PARATHYROID-HORMONE FRAGMENTS STIMULATE OSTEOCLAST-LIKE CELL-FORMATION AND OSTEOCLASTIC ACTIVITY

被引：59

作者：

KAJI, H ^{[1
]}

SUGIMOTO, T ^{[1
]}

KANATANI, M ^{[1
]}

MIYAUCHI, A ^{[1
]}

KIMURA, T ^{[1
]}

SAKAKIBARA, S ^{[1
]}

FUKASE, M ^{[1
]}

CHIHARA, K ^{[1
]}

机构：

[1] PEPTIDE INST INC,PROT RES FDN,OSAKA 562,JAPAN

来源：

ENDOCRINOLOGY | 1994年 / 134卷 / 04期

关键词：

D O I：

10.1210/en.134.4.1897

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The controversy still exists about the biological activity of carboxyl (C)-terminal PTH fragments. The present study was performed to examine the effect of C-terminal PTH fragments on osteoclast-like cell formation and bone-resorbing activity. In contrast to human (h) PTH(1-34) or hPTH-(1-84), any C-terminal fragments examined [hPTH(3-84), hPTH-(53-84), and hPTH-(69-84)] did not affect cellular cAMP production and intracellular calcium in osteoblastic UMR-106 cells. Although hPTH-(1-84) caused an increase in cAMP production and intracellular calcium less effectively than hPTH-(1-34) in UMR-106 cells, the former caused a stimulation of osteoclast-like cell formation in osteoblast-containing mouse bone cell cultures more effectively than the latter. All of the C-terminal fragments significantly stimulated osteoclast-like cell formation, and their effectiveness seemed to depend on the amino acid length of the fragments. The conditioned medium from UMR-106 cells pretreated with C-terminal PTH as well as amino-terminal PTH significantly stimulated osteoclast-like cell formation from mouse hemopoietic blast cells supported by granulocyte-macrophage colony-stimulating factor. Moreover, all of the C-terminal fragments stimulated osteoclast-like cell formation from hemopoietic blast cells even in the absence of osteoblasts, and their effectiveness seemed to depend on the length of fragments. As for bone-resorbing activity by mature osteoclasts, all of the C-terminal fragments stimulated bone resorption in osteoblast-containing mouse bone cell cultures, whereas these fragments did not affect the bone-resorbing activity of isolated rabbit osteoclasts. The present study first indicates that C-terminal PTH fragments stimulate osteoclast-like cell formation as weil as bone-resorbing activity by mature osteoclasts in the presence of osteoblasts and accelerate osteoclast-like cell formation from hemopoietic blast cells in the absence of osteoblasts.

引用

页码：1897 / 1904

页数：8

共 42 条

[1] EXPRESSION CLONING OF A COMMON RECEPTOR FOR PARATHYROID-HORMONE AND PARATHYROID HORMONE-RELATED PEPTIDE FROM RAT OSTEOBLAST-LIKE CELLS - A SINGLE RECEPTOR STIMULATES INTRACELLULAR ACCUMULATION OF BOTH CAMP AND INOSITOL TRISPHOSPHATES AND INCREASES INTRACELLULAR FREE CALCIUM [J].