ANTIOXIDATIVE ACTIVITY OF CHLOROPHYLL DERIVATIVES, OXYGEN STRESS RELIEVERS, TO SUPEROXIDE MEASURED BY USING CYPRIDINA LUCIFERIN ANALOGS

According to the Cypridina chemiluminescence method for measuring rate constants between superoxide (O2-) and antioxidants established in our previous report, the reaction constants between O2- and metallo-chlorophyllins or chlorins e6, whose oxidative stress relief activities at ischemia-reperfusion were inspected, were measured by quenching experiments of chemiluminescence of a Cypridina luciferin analogue, 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (CLA), in 25 mM buffer solutions (pH 7.0) at 25-degrees-C. The results were compared with known data in the literature, and showed that the present method can be used to measure reaction activities of antioxidative samples even if fairly unstable. The results show that oxidative stress relievers have strong antioxidative activity against in aqueous solutions.