LONG-RANGE INTERACTIONS IN MAMMALIAN PLATELET-AGGREGATION .2. THE ROLE OF PLATELET PSEUDOPOD NUMBER AND LENGTH

被引:31
作者
FROJMOVIC, M [1 ]
LONGMIRE, K [1 ]
VANDEVEN, TGM [1 ]
机构
[1] PULP & PAPER RES INST CANADA, DEPT PHYSIOL, MONTREAL H3A 2A7, QUEBEC, CANADA
基金
英国医学研究理事会;
关键词
D O I
10.1016/S0006-3495(90)82378-X
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In part 1, we reported that human (H) platelets, activated with high concentrations (10 microM) of adenosine diphosphate, aggregate under Brownian diffusion (nonstirred, platelet-rich plasma) with an apparent efficiency of collision (alpha B) approximately 4 times and 8 times larger than observed, respectively, for canine (C) and rabbit (R) platelets. Further evaluations of parallel inhibition of alpha B and shape change suggested a central role for platelet pseudopods in mediating the long-range interactions associated with the elevated alpha B values. We found that greater than 90% of all platelet contacts in the doublets and triplets formed were via at least one pseudopod. We therefore compared pseudopod number and length per platelet generated by approximately 30 s post ADP activation in nonstirred PRP from human, canine, and rabbit donors, using phase-contrast, video-enhanced microscopy of fixed platelets. Theoretical calculations assessing the effects of pseudopod length and number on the collision frequency enhanced by an increased radius of a collision sphere supported the experimental observations that approximately 3 or 4 pseudopods per human or canine platelet, and approximately 5 or 6 pseudopods per rabbit platelet yield optimal alpha B values, with the average pseudopod length: approximately 3:2:1 for H/C/R, paralleling the alpha B differences. After correcting for effects of pseudopods and platelet size on platelet diffusion and sedimentation, it still appeared that the small number of long pseudopods formed on human platelets could largely explain the unusually large alpha B values. The quantitative discrepancies between theory and experiment do not appear related to time-dependent refractoriness within the less than 60 s of observation, but may be related to biochemical differences in dynamics and surface density of adhesive (sticky sites) present on the pseudopod surface. © 1990, The Biophysical Society. All rights reserved.
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页码:309 / 318
页数:10
相关论文
共 24 条
[1]   TRANSFORMATION AND MOTILITY OF HUMAN-PLATELETS - DETAILS OF THE SHAPE CHANGE AND RELEASE REACTION OBSERVED BY OPTICAL AND ELECTRON-MICROSCOPY [J].
ALLEN, RD ;
ZACHARSKI, LR ;
WIDIRSTKY, ST ;
ROSENSTEIN, R ;
ZAITLIN, LM ;
BURGESS, DR .
JOURNAL OF CELL BIOLOGY, 1979, 83 (01) :126-142
[2]  
ASCH AS, 1985, BLOOD, V66, P926
[3]  
FILES JC, 1981, BLOOD, V58, P607
[4]  
FROJMOVIC MM, 1989, METHOD ENZYMOL, V169, P134
[5]   HUMAN-PLATELET SIZE, SHAPE, AND RELATED FUNCTIONS IN HEALTH AND DISEASE [J].
FROJMOVIC, MM ;
MILTON, JG .
PHYSIOLOGICAL REVIEWS, 1982, 62 (01) :185-261
[6]  
FROJMOVIC MM, 1983, BLOOD CELLS, V9, P359
[7]   GEOMETRY OF NORMAL MAMMALIAN PLATELETS BY QUANTITATIVE MICROSCOPIC STUDIES [J].
FROJMOVIC, MM ;
PANJWANI, R .
BIOPHYSICAL JOURNAL, 1976, 16 (09) :1071-1089
[8]  
FROJMOVIC MM, 1983, J LAB CLIN MED, V101, P964
[9]  
KARINO T, 1987, HEMOSTASIS THROMBOSI, P739
[10]  
MARSH SA, 1982, EUR J CELL BIOL, V28, P278