DIRECT IDENTIFICATION OF INDIVIDUAL VASOPRESSIN-CONTAINING NERVE-TERMINALS OF THE RAT NEUROHYPOPHYSIS AFTER WHOLE-CELL PATCH-CLAMP RECORDINGS

被引：21

作者：

WANG, XM ^{[1
]}

TREISTMAN, SN ^{[1
]}

LEMOS, JR ^{[1
]}

机构：

[1] WORCESTER FDN EXPTL BIOL INC,SHREWSBURY,MA 01545

来源：

NEUROSCIENCE LETTERS | 1991年 / 124卷 / 01期

关键词：

AVP; DOT IMMUNOBINDING; CALCIUM CURRENT; NERVE ENDING; POSTERIOR PITUITARY;

D O I：

10.1016/0304-3940(91)90838-K

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The membrane currents in rat neurohypophysial nerve terminals, which contain either vasopressin or oxytoxin, have been previously recorded using the "whole-cell" patch-clamp technique. Interpretation of the electrophysiological data would be significantly strengthened by the ability to correlate them with knowledge of the peptide contents of the terminals being studied. Here, a novel method for detection of the peptide hormone, arginine vasopressin, in those individual isolated terminals is described. The unique aspect of this procedure is that the contents of the terminal are aspirated into the recording electrode after 'whole-cell' patch-clamp recording, and then a highly sensitive dot immunobinding assay allows identification of the peptide contents in the terminals.

引用

页码：125 / 128

页数：4

共 19 条

[1] THE ROLE OF PATTERNED BURST AND INTERBURST INTERVAL ON THE EXCITATION-COUPLING MECHANISM IN THE ISOLATED RAT NEURAL LOBE [J].