HUMAN DECIDUA - A SOURCE OF CACHECTIN-TUMOR NECROSIS FACTOR

被引：73

作者：

ROMERO, R ^{[1
]}

MAZOR, M ^{[1
]}

MANOGUE, K ^{[1
]}

OYARZUN, E ^{[1
]}

CERAMI, A ^{[1
]}

机构：

[1] ROCKEFELLER UNIV,MED BIOCHEM LAB,NEW YORK,NY 10021

来源：

EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY | 1991年 / 41卷 / 02期

基金：

美国国家卫生研究院;

关键词：

DECIDUA; CACHECTIN-TUMOR NECROSIS FACTOR; PRETERM LABOR; ENDOTOXIN; INTRAUTERINE INFECTION; CHORIOAMNIONITIS; PREMATURITY;

D O I：

10.1016/0028-2243(91)90089-4

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Cachectin-tumor necrosis factor (TNF-alpha) has been implicated as a possible signal for the initiation of human parturition in the setting of infection. These studies were conducted to determine whether human decidua can produce TNF-alpha in response to bacterial lipopolysaccharide (LPS). Decidual explants from women undergoing elective cesarean sections were incubated with and without Escherichia coli LPS (25 ng/ml) for 20 h. TNF-alpha concentration in the conditioned media was measured with an enzyme-linked immunoassay and bioassay (L929 bioassay). While conditioned media from unstimulated decidual explants contained either undetectable or low levels of TNF-alpha, conditioned media from LPS stimulated decidua contained TNF-alpha (mean = 2.6 pmol/mg protein per 20 hours, SEM +/- 1.03). There was a strong correlation between the immunoreactive and bioactive TNF-alpha (Spearman rank correlation r = 0.76, P < 0.001). We conclude that human decidua in vitro can produce TNF-alpha in response to LPS.

引用

页码：123 / 127

页数：5

共 32 条

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[2]

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