THRESHOLD PHENOMENA AND LONG-DISTANCE ACTIVATION OF TRANSCRIPTION BY RNA POLYMERASE-II

被引：95

作者：

LAYBOURN, PJ

KADONAGA, JT

机构：

[1] UNIV CALIF SAN DIEGO, DEPT BIOL, LA JOLLA, CA 92093 USA

[2] UNIV CALIF SAN DIEGO, CTR MOLEC GENET, LA JOLLA, CA 92093 USA

来源：

SCIENCE | 1992年 / 257卷 / 5077期

关键词：

D O I：

10.1126/science.1388287

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To explore the underlying mechanisms by which genes are regulated in eukaryotes, long-distance transcriptional activation and threshold effects were reconstituted in vitro. Long-range activation of transcription by GAL4-VP16 protein located 1300 base pairs upstream of the RNA start site was dependent on packaging of the template into histone H1-containing chromatin. A transcriptional threshold effect by GAL4-VP16 was observed with repressed chromatin templates but not naked DNA templates. The experimental data with the chromatin templates were similar to the theoretical activation profile that is predicted if the action of each DNA bound protomer of GAL4-VP16 were independent and additive in terms of free energy.

引用

页码：1682 / 1685

页数：4

共 36 条

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