THE NEUROPEPTIDE VIP MODULATES THE NEUROTRANSMITTER PHENOTYPE OF CULTURED CHICK SYMPATHETIC NEURONS

被引：6

作者：

BERETTA, C ^{[1
]}

ZURN, AD ^{[1
]}

机构：

[1] INST PATHOL,DIV NEUROPATHOL,27 RUE BUGNON,CH-1011 LAUSANNE,SWITZERLAND

来源：

DEVELOPMENTAL BIOLOGY | 1991年 / 148卷 / 01期

关键词：

D O I：

10.1016/0012-1606(91)90319-X

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The present report provides evidence for a novel function for the neuropeptide vasoactive intestinal peptide (VIP). We demonstrate that VIP increases the cholinergic and the noradrenergic properties of cultured chick sympathetic neurons without changing neuronal survival and metabolism. VIP induces a 10- to 15-fold increase in the activity of choline acetyltransferase and an approximately twofold increase in the activity of tyrosine hydroxylase. Forskolin, an activator of adenylate cyclase, mimics all the effects of VIP on these cells. In addition, the effects of forskolin and VIP at optimal concentrations are not additive. Furthermore, VIP induces a rapid increase in the intracellular cAMP levels. Thus VIP acts via a cAMP-dependent pathway to enhance the cholinergic and noradrenergic properties of cultured chick sympathetic neurons. © 1991.

引用

页码：87 / 94

页数：8

共 62 条

[1] SYMPATHETIC NEURON DENSITY DIFFERENTIALLY REGULATES TRANSMITTER PHENOTYPIC-EXPRESSION IN CULTURE [J].

ADLER, JE ;

BLACK, IB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (12) :4296-4300

[2] PARTIAL-PURIFICATION AND CHARACTERIZATION OF A MEMBRANE-DERIVED FACTOR REGULATING NEUROTRANSMITTER PHENOTYPIC-EXPRESSION [J].

ADLER, JE ;

SCHLEIFER, LS ;

BLACK, IB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (03) :1080-1083

[3] PURIFICATION OF THE CHICK EYE CILIARY NEURONOTROPHIC FACTOR [J].

BARBIN, G ;

MANTHORPE, M ;

VARON, S .

JOURNAL OF NEUROCHEMISTRY, 1984, 43 (05) :1468-1478

[4] TRANSCRIPTIONAL REGULATION OF GROWTH-HORMONE GENE-EXPRESSION BY GROWTH HORMONE-RELEASING FACTOR [J].

BARINAGA, M ;

YAMONOTO, G ;

RIVIER, C ;

VALE, W ;

EVANS, R ;

ROSENFELD, MG .

NATURE, 1983, 306 (5938) :84-85

[5] NONNEURONAL CELLS MEDIATE NEUROTROPHIC ACTION OF VASOACTIVE-INTESTINAL-PEPTIDE [J].

BRENNEMAN, DE ;

NEALE, EA ;

FOSTER, GA ;

DAUTREMONT, SW ;

WESTBROOK, GL .

JOURNAL OF CELL BIOLOGY, 1987, 104 (06) :1603-1610

[6] INTERFERON INDUCES ASTROCYTE MATURATION CAUSING AN INCREASE IN CHOLINERGIC PROPERTIES OF CULTURED HUMAN SPINAL-CORD CELLS [J].

ERKMAN, L ;

WUARIN, L ;

CADELLI, D ;

KATO, AC .

DEVELOPMENTAL BIOLOGY, 1989, 132 (02) :375-388

[7] PROLIFERATION AND DIFFERENTIATION OF EMBRYONIC CHICK SYMPATHETIC NEURONS - EFFECTS OF CILIARY NEUROTROPHIC FACTOR [J].

ERNSBERGER, U ;

SENDTNER, M ;

ROHRER, H .

NEURON, 1989, 2 (03) :1275-1284

[8]

ETGEN AM, 1983, J NEUROSCI, V3, P2487

[9] RAPID RADIOCHEMICAL METHOD FOR DETERMINATION OF CHOLINE-ACETYLTRANSFERASE [J].

FONNUM, F .

JOURNAL OF NEUROCHEMISTRY, 1975, 24 (02) :407-409

[10] CALCITONIN GENE RELATED PEPTIDE AND MUSCLE-ACTIVITY REGULATE ACETYLCHOLINE-RECEPTOR ALPHA-SUBUNIT MESSENGER-RNA LEVELS BY DISTINCT INTRACELLULAR PATHWAYS [J].

FONTAINE, B ;

KLARSFELD, A ;

CHANGEUX, JP .

JOURNAL OF CELL BIOLOGY, 1987, 105 (03) :1337-1342

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