FUNCTIONAL-ANALYSIS OF THE MAJOR OUTER-MEMBRANE PROTEIN GENE PROMOTERS OF CHLAMYDIA-TRACHOMATIS

被引:15
作者
DOUGLAS, AL [1 ]
HATCH, TP [1 ]
机构
[1] UNIV TENNESSEE,DEPT MICROBIOL & IMMUNOL,MEMPHIS,TN 38163
关键词
D O I
10.1128/jb.177.21.6286-6289.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We determined that the putative upstream promoter of the long transcript (P1) of omp.4, the gene encoding the major outer membrane protein of Chlamydia trachomatis L2, is recognized in vitro by the major sigma factor of chlamydiae, sigma(66). We found no evidence that the putative downstream promoter of the short ompA transcript (P1) is functional in vitro. RNase protection of guanylyltransferase-capped transcripts made in vivo confirmed that P2 is a primary transcript while P1 is a processed product of a longer transcript, possibly P2.
引用
收藏
页码:6286 / 6289
页数:4
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