RELIABLE GOLGI-KOPSCH MODIFICATION

RILEY, J. N. A reliable Golgi-Kopsch modification. BRAIN RES. BULL. 4(1) 127-129, 1979.-A reliable Golgi-Kopsch variant is described. Tissue is fixed with 1% paraformaldehyde, 1% glutaraldehyde in 0.1 M phosphate buffer, pH 7.2. Tissue slabs (2.5-4 mm thick) are immersed for 5-7 days in a solution containing 3.5 g potassium dichromate, 15 g sucrose, 1.65 ml formaldehyde in 100 ml distilled water, washed briefly in 0.75% silver nitrate, and immersed in 0.75% silver nitrate for 2-3 days. Procedures for cutting, counterstaining, and handling sections are described. © 1979.