A MODIFIED METHOD FOR CHONDROSULFATASE ASSAY

被引：218

作者：

KAWAI, Y

SENO, N

ANNO, K

机构：

[1] Department of Chemistry, Faculty of Science, Ochanomizu University, Bunkyo-ku, Tokyo

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 32卷 / 02期

关键词：

D O I：

10.1016/0003-2697(69)90091-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The turbidimetric method of Dodgson and Price has been modified for chondrosulfatase assay. The method involves removal of undegraded chondroitin sulfates with cetylpyridinium chloride, modification of the barium chloride-gelatin reagent, and substitution of 0.2N hydrochloric acid for the 3% trichloroacetic acid of the original method. By using this modified method, the presence of chondrosulfatase activity was demonstrated in the liver of the squid, Ommastrephes sloani pacificus. © 1969.

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页码：314 / &

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