DIFFUSION IN GEL-ENZYME LINKED IMMUNOSORBENT ASSAY (DIG-ELISA) - SIMPLE METHOD FOR QUANTITATION OF CLASS-SPECIFIC ANTIBODIES

被引：68

作者：

ELWING, H ^{[1
]}

NYGREN, H ^{[1
]}

机构：

[1] GOTHENBURG UNIV, DEPT HISTOL, S-41346 GOTHENBURG, SWEDEN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 31卷 / 1-2期

关键词：

D O I：

10.1016/0022-1759(79)90290-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for quantifying class-specific antibodies is presented. The method has been named Diffusion-In-Gel-Enzyme-Linked-ImmunoSorbent Assay (DIG-ELISA), and is briefly as follows. Antiserum is allowed to diffuse from wells in a gel layered over an antigen-coated plastic surface. The gel is then removed and the preparation is incubated with enzyme-conjugated anti-immunoglobulin. The enzyme is then visualised in situ by a colour reaction produced by pouring a substrate-containing gel over the plastic surface. Bovine serum albumin and rabbit-anti-BSA were used as a model system, and horse-radish peroxidase or alkaline phosphatase as enzymes for visualization. © 1979.

引用

页码：101 / 107

页数：7

共 7 条

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