CLONING AND SEQUENCE DETERMINATION OF THE SCHIZOSACCHAROMYCES-POMBE RPB1 GENE ENCODING THE LARGEST SUBUNIT OF RNA POLYMERASE-II

被引：42

作者：

AZUMA, Y ^{[1
]}

YAMAGISHI, M ^{[1
]}

UESHIMA, R ^{[1
]}

ISHIHAMA, A ^{[1
]}

机构：

[1] NATL INST GENET, DEPT MOLEC GENET, MISHIMA, SHIZUOKA 411, JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 03期

关键词：

D O I：

10.1093/nar/19.3.461

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene, rpb1, encoding the largest subunit of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB1, of Saccharomyces cerevisiae as a cross-hybridization probe. We have determined the complete sequence of this gene, and parts of PCR-amplified rpb1 cDNA. The predicted coding sequence, interrupted by six introns, encodes a polypeptide of 1,752 amino acid residues in length with a molecular weight of 194 kilodaltons. This polypeptide contains eight conserved structural domains characteristic of the largest subunit of RNA polymerases from other eukaryotes and, in addition, 29 repetitions of the C-terminal heptapeptide found in all the eukaryotic RNA polymerase II largest subunits so far examined.

引用

页码：461 / 468

页数：8

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