ANALYSIS OF THE ENANTIOMERS OF FLUOXETINE AND NORFLUOXETINE IN PLASMA AND TISSUE USING CHIRAL DERIVATIZATION AND NORMAL-PHASE LIQUID-CHROMATOGRAPHY

被引：57

作者：

POTTS, BD

PARLI, CJ

机构：

[1] Department of Drug Metabolism and Disposition, Lilly Research Laboratories, Lilly Corporate Center

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 04期

关键词：

D O I：

10.1080/10826079208018825

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A stereospecific HPLC assay has been developed for the determination of the enantiomers of fluoxetine and an active metabolite, norfluoxetine, in plasma and tissue. Following derivatization with R-1-(1-napthyl)ethyl isocyanate, the diasteriomeric derivatives are resolved on a 5-mu-m silica column using a iso-octane/tetrahydrofuran mobile phase with fluorescence detection. The assay has a sensitivity of 5 ng/ml in plasma and 25 ng/gm in tissue. The calibration curves are linear over the range of 5-1000 ng/ml. The assay appears to be readily applicable to the study of enantioselective fluoxetine pharmacokinetics in animals and humans.

引用

页码：665 / 681

页数：17

共 27 条

[1] STEREOSELECTIVITY OF BIOACTIVE XENOBIOTICS - A PRE-PASTEUR ATTITUDE IN MEDICINAL CHEMISTRY, PHARMACOKINETICS AND CLINICAL-PHARMACOLOGY [J].