CONFOCAL AND FLUORESCENCE MICROSCOPIC STUDY USING LECTINS OF THE DISTRIBUTION OF CORTICAL GRANULES DURING THE MATURATION AND FERTILIZATION OF PIG OOCYTES

被引:81
作者
YOSHIDA, M
CRAN, DG
PURSEL, VG
机构
[1] USDA, INST LIVESTOCK & POULTRY SCI, REPROD LAB, BELTSVILLE, MD 20705 USA
[2] AFRC, INST ANIM PHYSIOL & GENET RES, DEPT MOLEC EMBRYOL, CAMBRIDGE CB2 4AT, ENGLAND
关键词
PEANUT AGGLUTININ; LASER MICROSCOPY; FITC-PNA;
D O I
10.1002/mrd.1080360409
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A study was carried out to determine whether pig cortical granules (CGs) could be visualized using fluorescein isothiocyanate (FITC)-labelled lectins. Following labelling with FITC-labelled peanut agglutinin (FITC-PNA), fluorescent spots were observed that had a distribution during maturation and fertilization entirely consistent with that observed by electron microscopy. For the first 18 h of in vitro maturation, most of the fluorescent spots of FITC-PNA were distributed throughout the cortical cytoplasm. Thereafter, the CGs underwent centrifugal migration to form a monolayer next to the plasma membrane. Following penetration by sperm, fluorescent spots were extruded into the perivitelline space, where they aggregated forming fluorescent clumps, which subsequently formed a reticulate structure surrounding the egg. Fluorescence was gradually lost such that by 18 h after insemination none could be detected in 70% of the eggs. The results indicate that CGs in pig oocytes contain galactosyl-rich glycoconjugates and that FITC-PNA is a useful probe for their rapid visualization and examination. (C) 1993 Wiley-Liss, Inc.
引用
收藏
页码:462 / 468
页数:7
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