HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR THE MEASUREMENT OF ATOVAQUONE IN PLASMA

被引：25

作者：

DEANGELIS, DV ^{[1
]}

LONG, JD ^{[1
]}

KANICS, LL ^{[1
]}

WOOLLEY, JL ^{[1
]}

机构：

[1] WELLCOME RES LABS,DIV PHARMACOKINET & DRUG METAB,3030 CORNWALLIS RD,RES TRIANGLE PK,NC 27709

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 652卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)E0387-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and efficient isocratic high-performance liquid chromatographic assay for the measurement of atovaquone in plasma has been developed and validated. The drug was extracted from plasma with organic solvents, assayed on a C1 column with a mobile phase of methanol-0.1% acetic acid (70:30, v/v), and detected by ultraviolet absorbance at 254 nm. Recovery of atovaquone from plasma was greater than 85%. Intra- and inter-assay variability were less than 8%, and the average accuracy of the assay (expressed as % bias) ranged from -7.4 to +2.2%. The upper and lower limits of quantitation were 100 and 0.25 mug/ml, respectively. Measurement of atovaquone in spiked plasma control samples during routine runs of clinical trial samples confirmed the reliability of the assay.

引用

页码：211 / 219

页数：9

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