XYLANOLYTIC ENZYME-PRODUCTION BY AN ASPERGILLUS-NIGER ISOLATE

被引：17

作者：

COSTAFERREIRA, M ^{[1
]}

DIAS, A ^{[1
]}

MAXIMO, C ^{[1
]}

MORGADO, MJ ^{[1
]}

SENAMARTINS, G ^{[1
]}

DUARTE, JC ^{[1
]}

机构：

[1] INETI,DTIQ,BIOTECHNOL UNIT,P-2745 QUELUZ,PORTUGAL

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1994年 / 44卷 / 03期

关键词：

ASPERGILLUS NIGER; XYLANASE; BETA-XYLOSIDASE; LOW CELLULASE PRODUCTION; PULP AND PAPER INDUSTRY APPLICATIONS;

D O I：

10.1007/BF02779659

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Production of xylanolytic enzymes by an Aspergillus niger CCMI 850 isolate was investigated in batch cultures. The effect of the composition of a fermentation medium that did not include chemical inducers, on beta-xylanase, beta-xylosidase, alpha-L-arabinofuranosidase, and total cellulase activity was studied. With 4% xylan as the carbon source, about 65 U/mL of beta-xylanase was obtained, whereas the total cellulase activity was undetectable, under the specified conditions. This beta-xylanase activity represents the highest reported for a wild-type strain of A. niger. The effect of pH and temperature on the activity of beta-xylanase was studied. Partial characterization of the beta-xylanase showed that with insoluble birchwood as substrate the K-m and Vmax were 0.3 mM and 19 mu mol/min, respectively. Aspects of using the crude beta-xylanase preparation for applications in the pulp and paper industry were discussed.

引用

页码：231 / 242

页数：12

共 22 条

[1]

BAILEY MJ, 1989, APPL MICROBIOL BIOT, V30, P5, DOI 10.1007/BF00255989

[2] MICROBIAL XYLANOLYTIC SYSTEMS [J].