A THIOREDOXIN FROM THE EXTREME THERMOPHILIC ARCHAEON SULFOLOBUS-SOLFATARICUS

被引：20

作者：

GUAGLIARDI, A

NOBILE, V

BARTOLUCCI, S

ROSSI, M

机构：

[1] UNIV NAPLES, DIPARTIMENTO CHIM ORGAN & BIOL, VIA MEZZOCANNONE 16, I-80134 NAPLES, ITALY

[2] CNR, IST BIOCHIM PROT & ENZIMOL, I-80125 NAPLES, ITALY

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1994年 / 26卷 / 03期

关键词：

D O I：

10.1016/0020-711X(94)90057-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. A purification procedure for a thioredoxin from the extremophilic archaeon Sulfolobus solfataricus is described. 2. The thioredoxin is active in the dithiothreitol-dependent reduction of insulin disulfide bonds. 3. The thioredoxin is a monomer of 24,800 Da; it is an acidic protein with a pI of 4.5. 4. The protein is stable to heating for 3 hr at 90-degrees-C. 5. The amino acid composition of S. solfataricus thioredoxin is reported.

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页码：375 / 380

页数：6

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