DETECTION OF DEOXYRIBONUCLEASE-I AND DEOXYRIBONUCLEASE-II (DNASE-I AND DNASE-II) ACTIVITIES IN REPRODUCTIVE-ORGANS OF MALE RABBITS

被引:28
作者
TAKESHITA, H [1 ]
YASUDA, T [1 ]
NADANO, D [1 ]
TENJO, E [1 ]
SAWAZAKI, K [1 ]
IIDA, R [1 ]
KISHI, K [1 ]
机构
[1] FUKUI MED SCH,DEPT LEGAL MED,FUKUI 91011,JAPAN
来源
INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1994年 / 26卷 / 08期
关键词
D O I
10.1016/0020-711X(94)90074-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. Deoxyribonucleases (DNases) I and II activities in 13 different organs and body fluids from healthy male rabbits were measured using the single radial enzyme diffusion method. 2. We now show that testis, epididymis, ampulla, seminal vesicle, vesicular gland, prostate, and semen have both of the DNases I and II activities, whereas spermatozoa do not. 3. DNase I activities were highest in epididymis and seminal vesicle, whereas DNase II activities were highest in epididymis and prostate among the reproductive organs. 4. The presence of these two enzyme activities outside the digestive system suggests that they have another biological function in addition to their digestive roles.
引用
收藏
页码:1025 / 1031
页数:7
相关论文
共 27 条
[1]  
Allan D.J., 1987, P229
[2]   ENDONUCLEASE ACTIVATION DURING APOPTOSIS - THE ROLE OF CYTOSOLIC CA2+ AND PH [J].
BARRY, MA ;
EASTMAN, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 186 (02) :782-789
[3]  
Bernardi G., 1971, HYDROLYSIS, P271
[4]  
EDDY EM, 1988, PHYSL REPRODUCTION, V1, P27
[5]  
KERR JFR, 1991, APOPTOSIS MOL BASIS, P5
[6]  
KISHI K, 1989, HUM GENET, V81, P295
[7]  
KISHI K, 1990, AM J HUM GENET, V47, P121
[8]   YOUNG AGE-RELATED GLYCOPROTEINS REVEALED IN NORMAL HUMAN-URINE BY ISOELECTRIC-FOCUSING [J].
KISHI, K ;
ISEKI, S .
PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 1982, 58 (07) :229-231
[9]  
Laskowski M., 1971, HYDROLYSIS, V4, P289
[10]  
MOORE S, 1981, ENZYMES A, V14, P281