A FLOW-INJECTION ANALYSIS SYSTEM INVOLVING IMMOBILIZED NADH OXIDASE IN COLUMN FORM FOR CLINICAL ANALYSIS

被引：6

作者：

MURACHI, T ^{[1
]}

TOTANI, M ^{[1
]}

IKEMOTO, M ^{[1
]}

TABATA, M ^{[1
]}

机构：

[1] KYOTO UNIV, COLL MED TECHNOL, KYOTO 606, JAPAN

来源：

JOURNAL OF BIOTECHNOLOGY | 1990年 / 14卷 / 01期

关键词：

Clinical analysis; FIA; Immobilized enzyme; Magnesium; NADH oxidase;

D O I：

10.1016/0168-1656(90)90015-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A highly sensitive FIA system for chemiluminometric determination of reduced coenzyme, NADH, was developed, using immobilized NADH oxidase from Brevibacterium ammoniagenes. The enzyme catalyzed the oxidation of NADH generating hydrogen peroxide which emitted chemiluminescence when mixed with luminol and potassium ferricyanide. The immobilized enzyme reactor was a mini-column, measuring 1 or 2 mm in inner diameter and 20 mm in length, and the sample volume was only 1 μl per assay, with a feeding speed of one sample per min and a lowest detection limit of 10 pmol NADH. A FIA system was also developed for the determination of magnesium in human serum, using an enzyme column reactor with simultaneously coimmobilized hexokinase, D-glucose-6-phosphate dehydrogenase, and NADH oxidase. The performance of the system was as satisfactory as a routine colorimetric assay, but with much higher sensitivity. © 1990.

引用

页码：33 / 41

页数：9

共 15 条

[1] CHEMILUMINESCENT ENZYME METHOD FOR GLUCOSE [J].