SOMATOSTATIN INHIBITION OF CA-2+-INDUCED INSULIN-SECRETION IN PERMEABILIZED HIT-T15 CELLS

被引:58
作者
ULLRICH, S [1 ]
PRENTKI, M [1 ]
WOLLHEIM, CB [1 ]
机构
[1] UNIV GENEVA,MED CTR,DIV BIOCHIM CLIN,9 AVE CHAMPEL,CH-1211 GENEVA 4,SWITZERLAND
关键词
D O I
10.1042/bj2700273
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Somatostatin inhibited Ca2+-induced insulin secretion in permeabilized HIT-T15 cells, albeit with decreased sensitivity relative to intact cells. The inhibitory action required the presence of GTP, whereas GDP could not substitute for GTP. Pertussis-toxin treatment before cell permeabilization abolished the inhibition of secretion. Thus somatostatin, by activating a G-protein, interferes with exocytosis distal to the generation of soluble intracellular messengers.
引用
收藏
页码:273 / 276
页数:4
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