COMPARISON OF DIFFERENT 2D-NMR TECHNIQUES FOR C-13, P-31 CORRELATION

Three different experimental techniques are compared to find the most efficient protocol for 2D C-13, P-31 correlation spectroscopy. It is shown that the best signal-to-noise ratio is given hy the polarization transfer experiment for P-31 detection and by the HMQC experiment for C-13 detection. Applications of these techniques for C-13, P-31 correlation of beta-nicotinamide adenine dinucleotide (NAD+) and a mixture of three different mononucleotides are described.