THE PROKARYOTIC ENHANCER BINDING-PROTEIN NTRC HAS AN ATPASE ACTIVITY WHICH IS PHOSPHORYLATION AND DNA-DEPENDENT

被引：146

作者：

AUSTIN, S

DIXON, R

机构：

[1] AFRC-IPSR Nitrogen Fixation Lab., University of Sussex

来源：

EMBO JOURNAL | 1992年 / 11卷 / 06期

关键词：

ATP HYDROLYSIS; DNA BINDING; PROTEIN PHOSPHORYLATION; TRANSCRIPTIONAL ACTIVATOR;

D O I：

10.1002/j.1460-2075.1992.tb05281.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The prokaryotic activator protein NTRC binds to enhancer-like elements and activates transcription in response to nitrogen limitation by catalysing open complex formation by sigma-54 RNA polymerase holoenzyme. Formation of open complexes requires the phosphorylated form of NTRC and the reaction is ATP dependent. We find that NTRC has an ATPase activity which is activated by phosphorylation and is strongly stimulated by the presence of DNA containing specific NTRC binding sites.

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页码：2219 / 2228

页数：10

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