CHARACTERIZATION OF THE CELLULAR-RESPONSE OF SPLEEN-CELLS IN BALB/C MICE INOCULATED WITH MYCOPLASMA-PNEUMONIAE OR THE P1-PROTEIN

BALB/c mice were intranasally infected or intraperitoneally inoculated with Mycoplasma pneumoniae whole cells or were immunized with the isolated adhesin (P1 protein). Spleen cells were isolated and tested in vitro for proliferation activity after stimulation with the P1 protein and sonicated M. pneumoniae whole antigen preparations. In frequency analysis experiments the P1 protein-specific proliferative response of spleen lymphocytes increased from 1/11494 in mice immunized once to 1/3246 in eightfold-inoculated mice, demonstrating that the P1 protein is a prominent immunogen of M. pneumoniae cells. Depletion experiments showed that T and B cells are activated in a 2:1 relation. Fluorescence-activated cells sorting analysis revealed a shift of the CD4/CD8 ratio from 2:1 in control mice up to 3:1 in M. pneumoniae-, and to 3.4:1 in P1 protein-immunized mice, as well as an increase in interleukin 2 receptor-bearing cells and macrophage cell populations. The results indicate that this animal model is appropriate to study host-M. pneumoniae interactions and vaccination schedules.