SIMULTANEOUS HPLC GRADIENT ANALYSIS OF 15 BENZODIAZEPINES AND SELECTED METABOLITES IN POSTMORTEM BLOOD

被引：55

作者：

MCINTYRE, IM ^{[1
]}

SYRJANEN, ML ^{[1
]}

CRUMP, K ^{[1
]}

HOROMIDIS, S ^{[1
]}

PEACE, AW ^{[1
]}

DRUMMER, OH ^{[1
]}

机构：

[1] MONASH UNIV,DEPT FORENS MED,S MELBOURNE,VIC 3205,AUSTRALIA

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 1993年 / 17卷 / 04期

关键词：

D O I：

10.1093/jat/17.4.202

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The simultaneous Identification and quantitation of 15 benzodiazepines and selected metabolites in postmortem blood, serum, or liver homogenate is described. The assay involves extraction with diethylether, followed by an acid clean-up step of the ether. Chromatographic separation was achieved on a Nova-Pak phenyl 18 column using ultraviolet detection at 240 nm. A gradient HPLC system was developed to improve separation of nitro-reduction metabolites from the solvent front and endogenous peaks. The mobile phases consisted of a gradient from 15 to 28% acetonitrile in 40mM potassium phosphate buffer. Within-run and day-to-day precision were generally 10–15%. The method described is sensitive and reproducible for the analysis of benzodiazepine concentrations in postmortem tissues. © 1993 Oxford University Press.

引用

页码：202 / 207

页数：6

共 42 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TRIAZOLAM IN HUMAN-SERUM [J].

ADAMS, WJ ;

RYKERT, UM ;

BOMBARDT, PA .

ANALYTICAL LETTERS PART B-CLINICAL AND BIOCHEMICAL ANALYSIS, 1980, 13 (02) :149-161

[2] SINGLE-DOSE AND MULTIPLE-DOSE KINETICS OF ESTAZOLAM, A TRIAZOLO BENZODIAZEPINE [J].

ALLEN, MD ;

GREENBLATT, DJ ;

ARNOLD, JD .

PSYCHOPHARMACOLOGY, 1979, 66 (03) :267-274

[3] CAPILLARY GAS-LIQUID CHROMATOGRAPHIC DETERMINATION OF THE BENZODIAZEPINE TRIAZOLAM IN PLASMA USING A RETENTION GAP [J].

BAKTIR, G ;

BIRCHER, J ;

FISCH, HU ;

KARLAGANIS, G .

JOURNAL OF CHROMATOGRAPHY, 1985, 339 (01) :192-197

[4] EMERGENCY TOXICOLOGICAL SCREENING FOR DRUGS COMMONLY TAKEN IN OVERDOSE [J].

BERRY, DJ ;

GROVE, J .

JOURNAL OF CHROMATOGRAPHY, 1973, 80 (02) :205-220

[5] SIMPLE AND SENSITIVE METHOD FOR MONITORING CLONAZEPAM IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

BOUKHABZA, A ;

LUGNIER, AA ;

KINTZ, P ;

MANGIN, P ;

CHAUMONT, AJ .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 529 (01) :210-216

[6] SIMULTANEOUS HPLC ANALYSIS OF THE HYPNOTIC BENZODIAZEPINES NITRAZEPAM, ESTAZOLAM, FLUNITRAZEPAM, AND TRIAZOLAM IN PLASMA [J].

BOUKHABZA, A ;

LUGNIER, AAJ ;

KINTZ, P ;

MANGIN, P .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1991, 15 (06) :319-322

[7] IDENTIFICATION AND QUANTITATION OF NEUTRAL AND BASIC DRUGS IN BLOOD BY GAS-CHROMATOGRAPHY AND MASS-SPECTROMETRY [J].

CAILLEUX, A ;

TURCANT, A ;

PREMELCABIC, A ;

ALLAIN, P .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1981, 19 (04) :163-176

[8]

de Boer A G, 1978, J Chromatogr, V145, P105, DOI 10.1016/S0378-4347(00)81672-2

[9] HYDROLYSIS OF CLONAZEPAM, FLUNITRAZEPAM AND NITRAZEPAM BY HYDROCHLORIC-ACID - IDENTIFICATION OF SOME ADDITIONAL PRODUCTS [J].

DEBRUYNE, MMA ;

SINNEMA, A ;

VERWEIJ, AMA .

FORENSIC SCIENCE INTERNATIONAL, 1984, 24 (02) :125-135

[10] A NEW METHOD FOR THE DETERMINATION IN BLOOD AND URINE OF A NOVEL TRIAZOLOBENZODIAZEPINE (ESTAZOLAM) BY HPLC [J].

DITELLA, AS ;

RICCI, P ;

DINUNZIO, C ;

CASSANDRO, P .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1986, 10 (02) :65-67

← 1 2 3 4 5 →