ANALYSIS AND CONFIGURATION ASSIGNMENTS OF THE AMINO-ACIDS IN A PYOVERDINE-TYPE SIDEROPHORE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：10

作者：

HANCOCK, DK

REEDER, DJ

机构：

[1] National Institute of Standards and Technology (NIST), Biotechnology Division, Gaithersburg

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 646卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)83346-T

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The amino acid composition of the siderophore pyoverdine Pf244 was determined by reversed phase high-performance liquid chromatography (RP-HPLC) of phenylthiocarbamyl derivatives of the acid-hydrolyzed pyoverdine. The siderophore contains an acid-labile amino acid, N(delta)-hydroxyornithine. and an amino acid previously unknown in naturally occurring systems, L-threo-beta-hydroxyhistidine. Amino acid chirality assignments were determined by RP-HPLC of the beta-D-glucopyranosyl isothiocyanate tetraacetate derivatives. Reactions with amino acid oxidases established the L-configuration of threo-beta-hydroxyhistidine.

引用

页码：335 / 343

页数：9

共 28 条

[1]

COHEN SA, 1989, PICO TAG METHOD MANU

[2] ASSIGNMENT OF THE CONFIGURATIONS OF THE AMINO-ACIDS IN PEPTIDIC SIDEROPHORES [J].

DEMANGE, P ;

ABDALLAH, MA ;

FRANK, H .

JOURNAL OF CHROMATOGRAPHY, 1988, 438 (02) :291-297

[3] BACTERIAL SIDEROPHORES - STRUCTURES OF PYOVERDINS-PT, SIDEROPHORES OF PSEUDOMONAS-TOLAASII NCPPB-2192, AND PYOVERDINS-PF, SIDEROPHORES OF PSEUDOMONAS-FLUORESCENS CCM-2798 - IDENTIFICATION OF AN UNUSUAL NATURAL AMINO-ACID [J].

DEMANGE, P ;

BATEMAN, A ;

MERTZ, C ;

DELL, A ;

PIEMONT, Y ;

ABDALLAH, MA .

BIOCHEMISTRY, 1990, 29 (50) :11041-11051

[4]

Demange P, 1987, IRON TRANSPORT MICRO, P167

[5] STRUCTURE OF FERRICHROME COMPOUNDS [J].

EMERY, T ;

NEILANDS, JB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1961, 83 (07) :1626-&

[6]

Greenstein J. P., 1961, CHEM AMINO ACIDS, V2

[7]

Greenstein J. P., 1961, CHEM AMINO ACIDS, V1

[8]

Greenstein J. P., 1961, CHEM AMINO ACIDS, V3

[9]

HANCOCK DD, UNPUB

[10] SYNTHESIS OF L-ERYTHRO-BETA-HYDROXYHISTIDINE FROM D-GLUCOSAMINE [J].

HECHT, SM ;

RUPPRECHT, KM ;

JACOBS, PM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1979, 101 (14) :3982-3983

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