BOVINE PROSTACYCLIN SYNTHASE - PURIFICATION AND ISOLATION OF PARTIAL CDNA

被引：17

作者：

PEREIRA, B ^{[1
]}

WU, KK ^{[1
]}

WANG, LH ^{[1
]}

机构：

[1] UNIV TEXAS,DEPT INTERNAL MED,VASC DIS RES CTR,HOUSTON,TX 77030

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 197卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.2583

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prostacyclin synthase (PGIS) from bovine aorta was purified using conventional purification procedures including detergent solubilization, Sephacryl S-300 gel filtration and Mono Q high performance liqud chromatography. Polyclonal antiserum raised against the 52-kDa protein bound specifically to PGIS. After immobilization to Protein A-sepharose, the PGIS-antibody complex displayed PGIS activity. Results based on (1) elution profile of the Mono Q column, (2) two-dimensional gel electrophoresis and (3) N-terminal amino acid sequence suggested that PGIS is heterogenous. Amino acid sequences of N-terminus and a tryptic peptide led us to isolate a partial cDNA fragment. © 1993 Academic Press. All rights reserved.

引用

页码：1041 / 1048

页数：8

共 17 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

DEWITT DL, 1983, J BIOL CHEM, V258, P3285

[3] ARTERIAL-WALLS ARE PROTECTED AGAINST DEPOSITION OF PLATELET THROMBI BY A SUBSTANCE (PROSTAGLANDIN-X) WHICH THEY MAKE FROM PROSTAGLANDIN ENDOPEROXIDES [J].

GRYGLEWSKI, RJ ;

BUNTING, S ;

MONCADA, S ;

FLOWER, RJ ;

VANE, JR .

PROSTAGLANDINS & OTHER LIPID MEDIATORS, 1976, 12 (05) :685-713

[4] PREPARATIVE HPLC PURIFICATION OF PROSTAGLANDIN ENDOPEROXIDES AND ISOLATION OF NOVEL CYCLOOXYGENASE-DERIVED ARACHIDONIC-ACID METABOLITES [J].

HECKER, M ;

HATZELMANN, A ;

ULLRICH, V .

BIOCHEMICAL PHARMACOLOGY, 1987, 36 (06) :851-855

[5] BOVINE ENDOTHELIAL-CELLS IN CULTURE PRODUCE THROMBOXANE AS WELL AS PROSTACYCLIN [J].

INGERMANWOJENSKI, C ;

SILVER, MJ ;

SMITH, JB ;

MACARAK, E .

JOURNAL OF CLINICAL INVESTIGATION, 1981, 67 (05) :1292-1296

[6]

Inoue M, 1987, Adv Prostaglandin Thromboxane Leukot Res, V17A, P29

[7]

KULMACZ RJ, 1987, PROSTAGLANDINS RELAT, P851

[8]

MATSUDAIRA P, 1987, J BIOL CHEM, V262, P10035

[9] DIFFERENTIAL FORMATION OF PROSTACYCLIN (PGX OR PGI2) BY LAYERS OF ARTERIAL-WALL - EXPLANATION FOR ANTI-THROMBOTIC PROPERTIES OF VASCULAR ENDOTHELIUM [J].

MONCADA, S ;

HERMAN, AG ;

HIGGS, EA ;

VANE, JR .

THROMBOSIS RESEARCH, 1977, 11 (03) :323-344

[10] A SIMPLIFIED ULTRASENSITIVE SILVER STAIN FOR DETECTING PROTEINS IN POLYACRYLAMIDE GELS [J].

OAKLEY, BR ;

KIRSCH, DR ;

MORRIS, NR .

ANALYTICAL BIOCHEMISTRY, 1980, 105 (02) :361-363

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