CANDIDA AND PSEUDOMONAS LIPASE-CATALYZED HYDROLYSIS OF BUTTEROIL IN THE ABSENCE OF ORGANIC-SOLVENTS

被引：18

作者：

MARANGONI, AG

机构：

[1] Dept. of Food Science, Univ. of Guelph, Guelph, Ontario

来源：

JOURNAL OF FOOD SCIENCE | 1994年 / 59卷 / 05期

关键词：

BUTTEROIL; HYDROLYSIS; FATTY ACIDS; LIPASE;

D O I：

10.1111/j.1365-2621.1994.tb08199.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Candida rugosa lipase (CRL) and Pseudomonas fluorescens lipase (PFL) were used to hydrolyze a low-melting point butteroil fraction in the absence of orgaic solvents. CRL and PFL displayed pH activity optima of 6.7 and 7.8, and temperature-activity optima of 40-50 degrees C and 35 degrees C, respectively. Maximum activity for PFL was observed at 5% (v/v) water content. CRL showed higher specificity towards butyric acid relative to PFL after 3 hours of hydrolysis at 40 degrees C (22.3 mol% vs 7.8 mol%, respectively). Both enzymes displayed, however, high specificities towards palmitic and oleic acids. CRL's inherent specificity towards butyric acid makes it an ideal choice for a biological catalyst to generate useful dairy flavor profiles.

引用

页码：1096 / 1099

页数：4

共 13 条

[1]

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[2] LIPASE-CATALYZED HYDROLYSIS OF MILK-FAT IN LECITHIN REVERSE MICELLES [J].