HORMONE-DEPENDENT TRANSACTIVATION BY THE HUMAN ANDROGEN RECEPTOR IS REGULATED BY A DNAJ PROTEIN

被引:102
作者
CAPLAN, AJ
LANGLEY, E
WILSON, EM
VIDAL, J
机构
[1] UNIV N CAROLINA, DEPT BIOCHEM & BIOPHYS, CHAPEL HILL, NC 27599 USA
[2] UNIV N CAROLINA, DEPT PEDIAT, CHAPEL HILL, NC 27599 USA
关键词
D O I
10.1074/jbc.270.10.5251
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genetic studies were performed to examine the role of eukaryotic dnaJ protein, Ydj1p, in the regulated activation of human androgen receptor (hAR) after heterologous expression in Saccharomyces cerevisiae. Hormone-dependent activation of hAR was measured as a function of lacZ reporter gene expression, which was defective in ydj1-151 and ydj1-2(Delta) null mutant strains compared to the wild type. This defect was not due to receptor misfolding, since hAR in both wild type and mutant strains had a similar capacity to bind hormone. The target for Ydj1p action was determined to be the hAR hormone binding domain since an N-terminal fragment lacking this region was constitutively active in both wild type and ydj1-151 mutant strains. These data correlate hormone dependence of hAR activation with a requirement for Ydj1p function and are consistent with a role for dnaJ proteins in signal transduction by steroid hormone receptors.
引用
收藏
页码:5251 / 5257
页数:7
相关论文
共 55 条