MOLECULAR-CLONING OF EQUINE INTERLEUKIN-1-ALPHA AND INTERLEUKIN-1-BETA CDNAS

被引：21

作者：

KATO, H ^{[1
]}

OHASHI, T ^{[1
]}

NAKAMURA, N ^{[1
]}

NISHIMURA, Y ^{[1
]}

WATARI, T ^{[1
]}

GOITSUKA, R ^{[1
]}

TSUJIMOTO, H ^{[1
]}

HASEGAWA, A ^{[1
]}

机构：

[1] UNIV TOKYO,FAC AGR,DEPT VET INTERNAL MED,BUNKYO KU,TOKYO 113,JAPAN

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1995年 / 48卷 / 3-4期

关键词：

INTERLEUKIN-1; HORSE; MOLECULAR CLONING;

D O I：

10.1016/0165-2427(95)05441-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Equine interleukin-1 alpha (IL-1 alpha) and IL-1 beta were molecularly cloned to establish a basis for research on inflammatory and immune responses in the horse. Equine peripheral blood mononuclear cells (PBMC) were stimulated with lipopolysaccharide (LPS), and cDNA clones of equine IL-1 alpha and IL-1 beta covering the whole coding sequences were isolated from them. These equine IL-1 alpha and IL-1 beta clones contained open reading frames encoding 271 and 269 amino acids, respectively. The deduced amino acid sequence of equine IL-1 alpha showed 71.6% and 60.2% similarity with that of human and murine IL-1 alpha, respectively. Similarly, the amino acid sequence of equine IL-1 beta showed 66.7% and 61.8% similarity with that of human and murine IL-1 beta, respectively. In both equine IL-1 alpha and IL-1 beta, amino acids at miristoylation sites were well conserved. Dot blot analysis indicated that the expression of IL-1 beta was predominant to that of IL-1 alpha in equine PBMC stimulated with LPS or phorbol myristate acetate.

引用

页码：221 / 231

页数：11

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