TETRASIALOGANGLIOSIDE GQ1B REACTIVE MONOCLONAL-ANTIBODIES - THEIR CHARACTERIZATION AND APPLICATION FOR QUANTIFICATION OF GQ1B IN SOME CELL-LINES OF NEURONAL AND ADRENAL ORIGIN(S)

被引:15
作者
YAMAMOTO, H
TSUJI, S
NAGAI, Y
机构
[1] UNIV TOKYO,FAC MED,DEPT BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN
[2] NIIGATA UNIV,BRAIN RES INST,DEPT NEUROL,NIIGATA 95021,JAPAN
关键词
Anti‐GQ1b monoclonal antibody; Neuroblastoma; Pheochromocytoma;
D O I
10.1111/j.1471-4159.1990.tb01901.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Seven monoclonal antibodies (MAbs) directed tetrasialoganglioside (GQ1b) were established, purified GQ1b being used for immunization and hybridoma screening. All of the MAbs reacted strongly with GQ1b, although they also reacted with other gangliosides, with different specificities and reactivities. Some MAbs (1H10, 2C7, and 3F4) reacted with GD3, GT1a, GQ1b, and GP1c. MAb 1H4 showed broad specificity. It reacted with GD3, GD1b, GD2, GT1a, GT1b, GQ1b, GQ1c, and GP1c. MAbs 7F5, 4E7, and 4F10 recognized GT1a, GQ1b, and GP1c. MAb 4F10 was more specific for GQ1b than the other MAbs. Using MAb 4F10, we determined, by means of an immunoassay, the quantities of endogenous GQ1b in some neuronal and adrenal cell lines, GOTO (human neuroblastoma), Neuro2a (mouse neuroblastoma), and PC12 (rat pheochromocytoma). PC12 and Neuro2a cells contained at least 5.1 X 106 and 3.9 X 105 molecules/cell of GQ1b, respectively. In contrast, no GQ1b was detected in GOTO cells, which are known for their specific neuritogenic response to this particular ganglioside when exogenously added. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码:513 / 517
页数:5
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