CYTOLOGIC ASSESSMENT OF TUMOR-CELL KINETICS - APPLICATIONS OF MONOCLONAL-ANTIBODY KI-67 TO FINE-NEEDLE ASPIRATION SMEARS

被引：15

作者：

HENRY, MJ ^{[1
]}

STANLEY, MW ^{[1
]}

SWENSON, B ^{[1
]}

CONNOLLY, JE ^{[1
]}

BATES, H ^{[1
]}

机构：

[1] HENNEPIN CTY MED CTR,DEPT PATHOL 815,701 PK AVE S,MINNEAPOLIS,MN 55415

来源：

DIAGNOSTIC CYTOPATHOLOGY | 1991年 / 7卷 / 06期

关键词：

CELL CYCLE; IMMUNOPEROXIDASE; NEOPLASMS; CYTOLOGY;

D O I：

10.1002/dc.2840070608

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Rates of tumor-cell proliferation often provide prognostic information about a given neoplasm. Previously available methods for accessing cell kinetics are time consuming and expensive, and often require special equipment or radioactive reagents. Monoclonal antibody Ki-67 binds a nuclear antigen expressed in proliferating but not in resting cells. We studied Ki-67 immunostaining of fine-needle aspiration smears from 40 benign and malignant masses. Labeling indices ranged from 0 (thyroid follicular adenoma) to 75 percent (pulmonary oat-cell carcinoma). Frozen section immunostaining (11 cases) and flow cytometric assessment of cell proliferation (8 cases) were in good agreement with Ki-67 labeling indices on smear material. We suggest that this method provides a rapid, inexpensive, and dependable means of assessing tumour-cell kinetics in cytologic preparations.

引用

页码：591 / 596

页数：6

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