A KINETOPLAST DNA PROBE DIAGNOSTIC FOR LEISHMANIA-INFANTUM

被引：19

作者：

GRAMICCIA, M

SMITH, DF

ANGELICI, MC

READY, PD

GRADONI, L

机构：

[1] NAT HIST MUSEUM,DEPT ENTOMOL,LONDON SW7 5BD,ENGLAND

[2] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOCHEM,LONDON SW7 2AY,ENGLAND

来源：

PARASITOLOGY | 1992年 / 105卷

基金：

英国医学研究理事会;

关键词：

LEISHMANIA-INFANTUM; KINETOPLAST DNA PROBE;

D O I：

10.1017/S0031182000073650

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A 196 bp fragment of cloned kinetoplast (k) DNA of Leishmania infantum has been sequenced and shown to be diagnostic for this species. The DNA from 10(4)-10(5) promastigotes was routinely and specifically detected in crude dot blots of whole cells lysed in situ, and there was no cross-hybridization with 10(6) cells of L. major, L. tropica, L. killicki, L. aethiopica, L. braziliensis and L. mexicana. A low degree of hybridization was found with L. donovani and L. chagasi. This probe will be of particular use in Mediterranean countries, such as Italy, in which dermotropic zymodemes of L. infantum can be difficult to isolate and grow in numbers sufficient for isoenzyme typing.

引用

页码：29 / 34

页数：6

共 25 条

[1] STUDY ON GENETIC-POLYMORPHISM OF LEISHMANIA-INFANTUM THROUGH THE ANALYSIS OF RESTRICTION ENZYME DIGESTION PATTERNS OF KINETOPLAST DNA [J].

ANGELICI, MC ;

GRAMICCIA, M ;

GRADONI, L .

PARASITOLOGY, 1989, 99 :301-309

[2]

Barker D. C., 1982, Biochemical characterization of Leishmania. Proceedings of a Workshop held at Pan American Health Organization, Washington, DC, 9-11 December 1980. [Editors: Chance, M.L.

[3]

Walton, B.C.], P139

[4]

BARKER DC, 1986, PARASITOLOGY, V91, pS139

[5]

BELAZZOUG S, 1985, ANN PARASIT HUM COMP, V60, P1

[6]

BULLOCK WO, 1987, BIOTECHNIQUES, V5, P376

[7]

Evans D.A., 1987, P52

[8]

FEINBERG AP, 1984, ANAL BIOCHEM, V137, P266

[9]

Gramiccia M., 1989, NATO ASI (Advanced Science Institutes) Series Series A Life Sciences, V163, P21

[10] LEISHMANIA INFANTUM SENSU-LATO AS AN AGENT OF CUTANEOUS LEISHMANIASIS IN ABRUZZI REGION (ITALY) [J].

GRAMICCIA, M ;

GRADONI, L ;

POZIO, E .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1987, 81 (02) :235-237

← 1 2 3 →