RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS OF TRYPANOSOMA-CRUZI STRAINS

被引:113
作者
STEINDEL, M [1 ]
NETO, ED [1 ]
DEMENEZES, CLP [1 ]
ROMANHA, AJ [1 ]
SIMPSON, AJG [1 ]
机构
[1] UNIV FED SANTA CATARINA,DEPT MICROBIOL & PARASITOL,BR-88000 FLORIANOPOLI,SC,BRAZIL
关键词
TRYPANOSOMA-CRUZI; GENOTYPE; RANDOM AMPLIFIED POLYMORPHIC DNA; ZYMODEME;
D O I
10.1016/0166-6851(93)90030-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
DNA extracted from 32 isolates of Trypanosoma cruzi was subjected to polymerase chain reaction amplification using 4 arbitrary primers resulting in relatively complex DNA profiles that include polymorphic markers known as random amplified polymorphic DNAs (RAPDs). The RAPD profiles of 18 strains belonging to zymodeme 1 (Z1) collected from various regions of South America exhibited a consistant pattern and 59 (59%) of the bands produced were present in all Z1 strains. A similar level of consistency was seen in the number of bands shared between 5 Z2 strains, 4 ZB strains and 2 ZC strains. A phenetic analysis of the 5 most different Z1 strains based on band sharing showed that their interrelationships mirrored their geographical origin. Comparison of the RAPD profiles of strains from different zymodemes showed that less than 7% of bands of strains in one zymodeme are present in strains of another zymodeme. Analysis of band sharing using bands present in all strains of a given zymodeme showed ZB and ZC to be closely related and Z1 and Z2 to form distinct groups.
引用
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页码:71 / 80
页数:10
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