EUROPIUM(III) ION PROBE SPECTROFLUOROMETRIC DETERMINATION OF DICLOFENAC SODIUM

被引:67
作者
CARREIRA, LA [1 ]
RIZK, M [1 ]
ELSHABRAWY, Y [1 ]
ZAKHARI, NA [1 ]
TOUBAR, SS [1 ]
机构
[1] UNIV MANSOURA,FAC PHARM,DEPT ANALYT CHEM,MANSOURA 35516,EGYPT
关键词
DICLOFENAC SODIUM; EUROPIUM(III) IONS; PHARMACEUTICAL; PROBE SPECTROFLUOROMETRY;
D O I
10.1016/0731-7085(95)01567-5
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new method has been devised for the determination of diclofenac sodium in bulk and in pharmaceutical preparations using Eu3+ ions as the Fluorescent probe. The technique was built around the hypersensitive property of the transitions of the fluorescent probe ion, Eu3+, at 616 nm. This is normally a forbidden transition, but the interaction with diclofenac sodium, which contains a carboxylic group, makes the transition allowed and enhances the intensity of its fluorescence emission. The Eu3+ fluorescence emission at 592 nm comes from a non-hypersensitive transition and is not affected by ligation. The intensity ratio, R, defined as I-592/I-616, was used as a measure of the percentage of bound probe ions. Diclofenac and Eu(III) forms a (1:1) molar complex. The relative stability constant of the complex was found to be 10(5). A linear relationship between bound Eu3+ and the concentration of diclofenac sodium was found for concentrations from 10 to 200 mu g ml(-1), with a recovery percentage of 100.22 +/- 2.27. The method shows a good agreement with a spectrophotometric method.
引用
收藏
页码:1331 / 1337
页数:7
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