INHIBIN SUPPRESSES HUMAN CHORIONIC-GONADOTROPIN SECRETION IN TERM, BUT NOT 1ST TRIMESTER, PLACENTA

被引:38
作者
MERSOLBARG, MS [1 ]
MILLER, KF [1 ]
CHOI, CM [1 ]
LEE, AC [1 ]
KIM, MH [1 ]
机构
[1] OHIO STATE UNIV, DEPT OBSTET & GYNECOL, COLUMBUS, OH 43210 USA
关键词
D O I
10.1210/jcem-71-5-1294
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Inhibin is produced by the placenta, with serum concentrations rising throughout pregnancy. In contrast, hCG serum concentrations peak in the first trimester and are 80–90% lower at term. This study was designed to determine the effect of inhibin on hCG secretion both early and late in gestation. Villus tissue from 3 term and 3 first trimester (8–10 week) placentas was maintained in an in vitro explant culture model for 5 days. Tissue from each placenta was incubated with control medium in 24 replicate wells for the first 72 h. During the final 48 h, 12 wells received control medium, and 12 wells received medium containing 1% rabbit antiserum raised against the asubunit (residues 1–32) of the human inhibin peptide. The antiserum demonstrated biological activity by increasing serum FSH concentrations in an immature female rat bioassay. The relative increase in hCG secretion at the conclusion of days 4 and 5 in control and antiserum-treated groups for each first trimester and term placenta were compared to pretreatment hCG concentrations on day 3. The relative increases in hCG secretion of first trimester control groups on day 4 (mean ± sd, 34 ± 11%) and day 5 (63 ± 23%) were compared to those in antiserum-treated groups on day 4 (39 ± 13%) and day 5 (54 ± 5%) and showed no significant difference between groups on either day. The same comparison in term cultures showed the relative increases in hCG secretion of control groups on day 4 (31 ± 10%) and day 5 (64 ± 50%) to be significantly lower than those in antiserum-treated groups on day 4 (100 ± 41%) and day 5 (150 ± 108%; P < 0.001). These findings suggest that inhibin suppresses hCG secretion in term, but not first trimester, placentas. © 1990 by The Endocrine Society.
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页码:1294 / 1298
页数:5
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