SPIN-LABELING OF SIALIC-ACID IN SOLUBLE AND CELL-SURFACE GLYCOPROTEINS

被引：7

作者：

APLIN, JD ^{[1
]}

BROOKS, DE ^{[1
]}

CULLING, CFA ^{[1
]}

HALL, LD ^{[1
]}

REID, PE ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,FAC MED,DEPT PATHOL,VANCOUVER V6T 1W5,BC,CANADA

来源：

CARBOHYDRATE RESEARCH | 1979年 / 75卷 / OCT期

关键词：

D O I：

10.1016/S0008-6215(00)84622-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new, mild method is described for spin-labelling sialic acid residues in situ. The procedure involves the formation of C-1 sialamides and has been applied to a serum glycoprotein, a mucin, tissue sections from human colon, and erythrocyte membrane components. The selectivity of the method and its possible applicability to other types of labelling are discussed. © 1979.

引用

页码：11 / 16

页数：6

共 20 条

[1] ASHWELL G, 1974, ADV ENZYMOL RAMB, V41, P99
[2] SPECIFICITY OF NEURAMINIDASE - SYNTHESIS OF 5-N-ACETYL-D-NONULOSAMINE (5-ACETAMIDO-3,5-DIDEOXY-D-GLYCERO-BETA-D-GALACTO-NONULOSE) AND 5-N-ACETYL-D-NEURAMINAMIDE (5-ACETAMIDO-3,5-DIDEOXY-D-GLYCERO-BETA-D-GALACTO-NONULOSONAMIDE) AND THEIR METHYL BETA-KETOSIDES AND BENZYL ALPHA-KETOSIDES
BROSSMER, R
HOLMQUIST, L
[J]. HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1971, 352 (12): : 1715 - +
[3] SIALOMUCOPEPTIDE LIBERATED BY TRYPSIN FROM THE HUMAN ERYTHROCYTE
COOK, GMW
HEARD, DH
SEAMAN, GVF
[J]. NATURE, 1960, 188 (4755) : 1011 - 1012
[4] 6-ALPHA-D-SIALYL-N-ACETYLGALACTOSAMINE - THE NEURAMINIDASE-SUSCEPTIBLE PROSTHETIC GROUP OF BOVINE SALIVARY MUCOPROTEIN
GOTTSCHALK, A
GRAHAM, ERB
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1959, 34 (02) : 380 - 391
[5] ISOLATION AND REACTIVITY OF A MODEL FOR CARBODIIMIDE-CARBOXYLIC ACID ADDUCT - O-BENZOYL-N,N-DIMETHYL-N'-(N-METHYL-2,4-DINITROANILINO)ISOUREA
HEGARTY, AF
MCCORMACK, MT
FERGUSON, G
ROBERTS, PJ
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1977, 99 (06) : 2015 - 2016
[6] JOURDIAN GW, 1971, J BIOL CHEM, V246, P430
[7] USE OF SPIN LABEL TEMPAMINE FOR MEASURING INTERNAL VISCOSITY OF RED BLOOD-CELLS
MORSE, PD
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1977, 77 (04) : 1486 - 1491
[8] NEUBERGER A, 1972, BIOCHEM J, V129, P683, DOI 10.1042/bj1290683
[9] PHYSICAL AND CHEMICAL STUDIES ON GLYCOPROTEINS .3. MICROHETEROGENEITY OF FETUIN A FETAL CALF SERUM GLYCOPROTEIN
OSHIRO, Y
EYLAR, EH
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1968, 127 (1-3) : 476 - &
[10] SIMPLE METHOD FOR DETERMINATION OF O-ACETYL SUBSTITUTION PATTERN OF SIALIC ACIDS OF COLONIC EPITHELIAL GLYCOPROTEIN
REID, PE
CULLING, CFA
RAMEY, CW
DUNN, WL
CLAY, MG
[J]. CANADIAN JOURNAL OF BIOCHEMISTRY, 1977, 55 (05): : 493 - 503

← 1 2 →