ISOLATION OF ACANTHAMOEBA SP FROM A GREYHOUND WITH PNEUMONIA AND GRANULOMATOUS AMEBIC ENCEPHALITIS

被引：21

作者：

BAUER, RW

HARRISON, LR

WATSON, CW

STYER, EL

CHAPMAN, WL

机构：

[1] Athens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Georgia, Athens

[2] Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens

[3] Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia, Tifton, GA 31793

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1993年 / 5卷 / 03期

关键词：

D O I：

10.1177/104063879300500314

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Acanthamoeba were isolated from a naturally occurring animal infection of granulomatous amebic encephalitis. The amebas were grown from lung lesions from a 1-year-old greyhound puppy, which was 1 of several dogs in a kennel that was affected by a progressive fatal neurologic and respiratory disease. The Centers for Disease Control, Atlanta, Georgia, confirmed the disease to be acanthamebiasis and specifically identified the amebas as Acanthamoeba culbertsoni by fluorescent antibody testing on brain tissue from the dog. The amebas were cultured initially on potato dextrose apr and on nonnutrient agar plates that were seeded with a lawn of nonpathogenic Escherichia coli. The isolate was then transferred to nonnutrient agar plates containing killed Enterobacter aerogenes and subsequently to axenic medium and cell cultures. The isolate was highly pathogenic by intranasal inoculation into 2-week-old mice.

引用

页码：386 / 391

页数：6

共 39 条

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