L-LYSINE - ALPHA-KETOGLUTARATE AMINOTRANSFERASE .I. IDENTIFICATION OF A PRODUCT DELTA1-PIPERIDEINE-6-CARBOXYLIC ACID

被引:95
作者
SODA, K
MISONO, H
YAMAMOTO, T
机构
[1] Institute for Chemical Research, Kyoto University, Uji City, Kyoto-Fu
关键词
D O I
10.1021/bi00851a045
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The product derived from L-lysine was isolated from the L-lysine-α-ketoglutarate aminotransferase system of Flavobacterium fuscum and its properties were studied. Radioactivity from DL-[l-14C]lysine was incorporated exclusively into a product which reacted with o-aminobenzaldehyde. Chromatographic and electrophoretic studies, characterization of the bisulfite and o-aminobenzaldehyde adducts and the condensation product, and comparison with authentic Δ1-piperideine-2-carboxylic acid offer evidence that the product is Δ1-piperideine-β-carboxylic acid. In this aminotransferase reaction, e-amino group of L-lysine is transferred to α-ketoglutarate to yield glutamate and α-aminoadipate-δ-semialdehyde which is immediately converted into the intramolecularly dehydrated form, Δ 1-piperideine-6-carboxylic acid. © 1968, American Chemical Society. All rights reserved.
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页码:4102 / &
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