HYDROLYSIS OF ESTER-TYPE DRUGS BY THE PURIFIED ESTERASE FROM HUMAN INTESTINAL-MUCOSA

被引：35

作者：

INOUE, M ^{[1
]}

MORIKAWA, M ^{[1
]}

YAMADA, T ^{[1
]}

SUGIURA, M ^{[1
]}

TSUBOI, M ^{[1
]}

机构：

[1] GIFU COLL PHARM,DEPT PHARM,GIFU,GIFU 502,JAPAN

来源：

JAPANESE JOURNAL OF PHARMACOLOGY | 1979年 / 29卷 / 01期

关键词：

D O I：

10.1254/jjp.29.17

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Esterase from human intestinal mucosa was purified 210 fold by solubilization with Triton X-100, chromatography on DEAF-cellulose, Sephadex G-100 and hydroxy lapatite, and isoelectric focusing. The purified esterase showed a single band by polyacrylamide gel electrophoresis. The molecular weight of the purified esterase was estimated to be about 55,000 by gel filtration on Sephadex G-150, and the isoelectric point was 5.02. The purified esterase was strongly inhibited by diethyl p-nitrophenyl phosphate (E-600) and diisopropyl fluorophosphate (DFP), and was not inhibited by eserine sulfate and p-chloromercuribenzoate. The purified esterase from human intestinal mucosa was found to be one of the carboxylesterases. The purified esterase hydrolyzed ester-type drugs, i.e., aspirin, clofibrate, indanyl carbenicillin and procaine, but did not hydrolyze amide-type drugs and choline-type drugs. © 1979, The Japanese Pharmacological Society. All rights reserved.

引用

页码：17 / 25

页数：9

共 16 条

[1]

AUGUSTINSSON KB, 1954, METHOD BIOCH ANAL, V5, P1

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