PURIFICATION AND CLONING OF A RIBOSOMAL-RNA GENE FRAGMENT FROM MOUSE DNA

被引：7

作者：

MISHIMA, Y ^{[1
]}

SAKAI, M ^{[1
]}

MURAMATSU, M ^{[1
]}

KATAOKA, T ^{[1
]}

HONJO, T ^{[1
]}

机构：

[1] UNIV TOKYO, FAC MED, DEPT PHYSIOL CHEM & NUTR, TOKYO 113, JAPAN

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 1978年 / 54卷 / 10期

关键词：

D O I：

10.2183/pjab.54.657

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Newborn mouse DNA was digested with a restriction endonuclease EcoRI and concentrated with respect to ribosomal RNA sequences by an RPC-5 column. DNA fragments of 14–17 kilobases in length, most probably containing promoter region of the ribosomal RNA gene, were used for cloning with λgt WES · λB as a vector using an in vitro packaging technique. Several clones containing 18S rRNA sequences were obtained. One of the clones which was transferred to a plasmid pBR322 (designated as pMrEL-1) was 14 kilobases in length, having only a part of 18S rRNA sequence. These results strongly suggest that this fragment carries a promoter region of the ribosomal RNA gene. © 1978, The Japan Academy. All rights reserved.

引用

页码：657 / 662

页数：6

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