ISOLATION AND CULTURE OF PROTOPLASTS FROM MOSS PROTONEMATA

被引：16

作者：

MAEDA, M

机构：

[1] Biological Laboratory, Faculty of Education, Fukui University, Fukui

来源：

BOTANICAL MAGAZINE-TOKYO | 1979年 / 92卷 / 1026期

关键词：

D O I：

10.1007/BF02493383

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Protoplats were enzymatically prepared from protonemata of the moss Plagiomnium vesicatum, which was cultured on a Murashige-Skoog's medium supplemented with 0.2% yeast extract and solidified with agar. The protoplasts incubated in soft agar medium containing sucrose began to divide after 15 days of incubation, and after 50 days 10- to 15-cell aggregates were present. When transferred to a sugar-free medium, the cells in the aggregates grew into filaments. © 1979 The Botanical Society of Japan.

引用

页码：105 / 110

页数：6

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