EFFECT OF GIBBERELLIC-ACID, (2-CHLOROETHYL) PHOSPHONIC ACID, ACTINOMYCIN-D AND CYCLOHEXIMIDE ON THE ACTIVITY AND LEACHING OF SOME HYDROLASES IN POLLEN SUSPENSION CULTURES OF CROTALARIA-JUNCEA

被引：10

作者：

BHANDAL, IS

MALIK, CP

机构：

[1] Department of Botany, College of Basic Sciences, Punjab Agricultural University, Ludhiana

来源：

PHYSIOLOGIA PLANTARUM | 1979年 / 45卷 / 03期

关键词：

D O I：

10.1111/j.1399-3054.1979.tb02586.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Gibberellic acid (GA3) 20 mg 1−1 and (2‐chloroethyl) phosphonic acid (Ethephon) 10 mg 1−1 enhanced pollen tube length in Crotalaria juncea L. GA3 markedly increased the activity of amylase, acid phosphatase and β‐glucosidase and enhanced leaching of amylase and acid phosphatase enzymes. The rise in the activity level and leaching of amylase and acid phosphatase after Ethephon treatment was comparatively less than that of GA3 and the response to Ethephon was restricted to the 45 and 90 min period of culturing. Ethephon did not affect the activity/leaching of β‐glucosidase significantly. Actinomycin‐D (Act.D) 25 mg l−1 and Cycloheximide (CH) 10 mg 1−1 reduced the tube growth as well as activity of these enzymes suggesting their de novo synthesis during pollen tube growth. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：297 / 300

页数：4

共 20 条

[1]

BAILEY KM, 1976, J EXP BOT, V27, P326

[2] GIBBERELLIC ACID-ENHANCED SYNTHESIS AND RELASE OF ALPHA-AMYLASE AND RIBONUCLEASE BY ISOLATED BARLEY ALEURONE LAYERS [J].