SACCHARIFICATION OF STEAM-EXPLODED POPLAR WOOD

被引:102
作者
EXCOFFIER, G [1 ]
TOUSSAINT, B [1 ]
VIGNON, MR [1 ]
机构
[1] CNRS,CERMAV,BP 53X,F-38041 GRENOBLE,FRANCE
关键词
TRICHODERMA-REESEI CL-847; STEAM EXPLOSION TREATMENT; SACCHARIFICATION; INACTIVATION; CELLULOSE; HEMICELLULOSES; LIGNIN;
D O I
10.1002/bit.260381108
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Effects of time, temperature, and pH during the steam explosion of poplar wood were studied with the aim of optimize both pentoses recovery and enzymatic hydrolysis efficiency. Steam explosion of acid impregnated wood chips allowed the recovery of 70% of potential xylose as monomers (217-degrees-C, 120 s). Enzymatic hydrolysis of pretreated fiber with Trichoderma reesei CL-847 cellulase system increased progressively with the severity of the steam treatment conditions. The best yield in term of glucose recovery after 24 h of enzymatic hydrolysis was 70% of potential glucose (225-degrees-C, 120 s). Deactivation by adsorption on lignin of Trichoderma reesei cellulases and inhibition of these enzymes by low-molecular-weight phenols and trihydroxybutyric acids were noticed.
引用
收藏
页码:1308 / 1317
页数:10
相关论文
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