RECEPTOR-STIMULATED ACCUMULATION OF PHOSPHATIDYLINOSITOL (3,4,5)-TRISPHOSPHATE BY G-PROTEIN MEDIATED PATHWAYS IN HUMAN MYELOID DERIVED CELLS

被引:154
作者
STEPHENS, L
EGUINOA, A
COREY, S
JACKSON, T
HAWKINS, PT
机构
[1] CSIC, CTR INVEST BIOL, E-28006 MADRID, SPAIN
[2] UNIV PITTSBURGH, DEPT PEDIAT, PITTSBURGH, PA 15213 USA
关键词
G-PROTEINS; NEUTROPHILS; P53-P56(LYN); PI3K; PI3-KINASE; PTDINS(3,4,5)P3;
D O I
10.1002/j.1460-2075.1993.tb05880.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phosphoinositide 30H-kinase (PI3K) activities are thought to be critical regulatory enzymes in a new intracellular signalling pathway, the activation of which results in the rapid accumulation of a putative signalling molecule, phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5) P3]. To date, activation of PI3K has always correlated with its recruitment into complexes containing protein tyrosine kinases (PTK). Here we report that agonists which utilize G-protein mediated transduction pathways can stimulate very rapid and large accumulations of PtdIns(3,4,5)P3 via a novel mechanism, possibly involving direct coupling between the G-protein and a PI3K activity. In addition, some of these agonists also stimulate small increases in PI3K activity in anti-phosphotyrosine and anti-src-type PTK antibody directed immunoprecipitates, indicating activation of PI3K via a 'conventional' PTK mediated mechanism; these pathways however, play only a minor role in the initial, agonist sensitive production of PtdIns(3,4,5)P3 in myeloid derived cells.
引用
收藏
页码:2265 / 2273
页数:9
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