SEX-HORMONE BINDING GLOBULIN PHENOTYPES - THEIR DETECTION AND DISTRIBUTION IN HEALTHY-ADULTS AND IN DIFFERENT CLINICAL CONDITIONS

被引:8
作者
CORNELISSE, MM
BENNETT, PE
CHRISTIANSEN, M
BLAAKAER, J
ANDERSEN, JR
HOMANN, C
GARRED, P
GLUUD, C
机构
[1] STATENS SERUM INST,DEPT CLIN BIOCHEM,DK-2300 COPENHAGEN,DENMARK
[2] RIGSHOSP,DEPT OBSTET & GYNECOL,DK-2100 COPENHAGEN,DENMARK
[3] RIGSHOSP,TISSUE TYPING LAB,DK-2100 COPENHAGEN,DENMARK
[4] HVIDOVRE UNIV HOSP,DEPT MED,DIV HEPATOL,COPENHAGEN,DENMARK
[5] KOMMUNE HOSP COPENHAGEN,INST PREVENT MED,COPENHAGEN,DENMARK
[6] CENT HOSP,DEPT MED,NYKOBING,DENMARK
[7] BISPEBJERG HOSP,DEPT MED B,DK-2400 COPENHAGEN,DENMARK
关键词
SEX HORMONE BINDING GLOBULIN PHENOTYPES; CARBOHYDRATE HETEROGENEITY; ELECTROPHORESIS; OVARIAN TUMOR; HIRSUTISM; LIVER CIRRHOSIS; ALCOHOLISM;
D O I
10.1016/0009-8981(94)90039-6
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Human sex hormone binding globulin (SHBG) is encoded by a normal and a variant allele. The resulting SHBG phenotypes (the homozygous normal SHBG, the heterozygous SHBG and the homozygous variant SHBG phenotype) can be distinguished by their electrophoretic patterns. We developed a novel detection system allowing us to distinguish between the different electrophoretic patterns in small amounts of plasma or serum (10 mu l). Small aliquots of Blue Sepharose were added to diluted sera or plasma: samples for removal of albumin and the supernatants were subsequently applied to Western blotting. This method of detection was used to determine the distribution of SHBG phenotypes:in healthy controls of both sexes and in five different pathological conditions characterized by changes in the SHBG level or endocrine disturbances (malignant and benign ovarian neoplasms, hirsutism, liver cirrhosis and alcoholism). The distribution of SHBG phenotypes was independent of such conditions and in agreement with the expected phenotype distribution of a bi-allelic gene in both healthy controls and patients (Hardy Weinberg law). An allele frequency of 0.13 was found for the SHBG variant allele based on the experimental values. Differences in SHBG phenotypes do not appear to have any clinical significance and no sex difference was found in the SHBG phenotype distribution.
引用
收藏
页码:115 / 121
页数:7
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