LOCALIZATION OF PROTEIN DISULFIDE-ISOMERASE TO THE EXTERNAL SURFACE OF THE PLATELET PLASMA-MEMBRANE

被引:167
作者
ESSEX, DW [1 ]
CHEN, K [1 ]
SWIATKOWSKA, M [1 ]
机构
[1] SUNY HLTH SCI CTR, DEPT MED, DIV HEMATOL ONCOL, BROOKLYN, NY 11203 USA
关键词
D O I
10.1182/blood.V86.6.2168.bloodjournal8662168
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Protein disulfide isomerase (PDI) is an enzyme that catalyzes the formation as well as the isomerization of disulfide bonds. In this study, antibodies against PDI were used to show PDI antigen on the platelet surface by indirect immunofluorescence microscopy and by flow cytometry. The platelets were not activated, as evidenced by the absence of staining by an antibody against P-selectin. Permeabilized platelets showed little cytosolic PDI by indirect immunofluorescence microscopy, suggesting that the majority of platelet PDI is localized to the platelet surface. PDI activity against ''scrambled'' RNase was shown with intact platelets. The activity was inhibited by inhibitors of PDI and by an antibody against PDI. Other blood cells showed little PDI, Platelet surface PDI may play a role in the various physiological and pathophysiologic processes in which platelets are involved. (C) 1995 by The American Society of Hematology.
引用
收藏
页码:2168 / 2173
页数:6
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