PERFORMANCE OF RECOMBINANT FERMENTATION AND EVALUATION OF GENE-EXPRESSION EFFICIENCY FOR GENE-PRODUCT IN 2-STAGE CONTINUOUS CULTURE SYSTEM

In order to develop a general methodology for evaluation of the gene expression efficiency for gene product, theoretical and experimental studies were undertaken using a recombinant Escherichia coli K12 DELTA H1 DELTA trp/pPLc23trp A1 as a 'gene-host cell' model system in a two-stage continuous-culture system. For this, a genetically structured kinetic model proposed earlier for biosynthesis of gene product in batch cultivation was extended to the two-stage continuous-culture system. A partial list of key parameters of the model includes the rate of plasmid segregation, specific growth rate of recombinant cell, plasmid content, rates of the transcription and translation, and other parameters related to product biosynthesis. The dynamics of heterogeneous cell population containing plasmid-harboring and plasmid-free cells were also studied.