CONTAMINATION OF DFP-TREATED LEUCINE AMINOPEPTIDASE WITH MULTIPLE ENDOPEPTIDASES

被引：9

作者：

DEFTOS, LJ

POTTS, JT

机构：

[1] Section on Polypeptide Hormones, Laboratory of Molecular Diseases, National Heart Institute, Bethesda, MD

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 171卷 / 01期

关键词：

D O I：

10.1016/0005-2744(69)90111-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1.Commercial preparations of leucine aminopeptidase (EC 3.4.1.1) were found to be contaminated with multiple endopeptidases of high molecular weight and varying substrate specificity. 2. 2.These contaminating endopeptidases could not be completely eliminated from the leucine aminopeptidase preparations with endopeptidase inhibitors or by further purification procedures. 3. 3.These findings emphasize the importance of testing for non-specific proteases in any preparation of leucine aminopeptidase to be used for the limited digestion of proteins or peptides. © 1969.

引用

页码：121 / &

共 11 条

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